Cytochalasin b glucose uptake

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August undefined, 2024

WebMay 9, 2024 · We artificially blocked the RBC GLUT1 transporter using either one of two routinely used GLUT1 inhibitors: cytochalasin B (CCB) and forskolin. These inhibitors bind GLUT1 in the transmembrane region, effectively blocking RBC D-glucose uptake (Guizouarn and Allegrini 2024 ). WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. This coincided with a marked decrease in D - [U- 14 C] glucose oxidation and suppression of the stimulant action of D -glucose upon insulin release.

Bioluminescent-based imaging and quantification …

WebJan 1, 1990 · Cytochalasin B also blocked the uptake of 14 C-L-ascorbic acid (1.25 mg%). The results of these studies further support the hypothesis that glucose and ascorbate … WebThe widely expressed mammalian glucose transporter, GLUT1, can be acutely activated in L929 fibroblast cells by a variety of conditions, including glucose deprivation, or treatment with various respiration inhibitors.Known thiol reactive compounds including phenylarsine oxide and nitroxyl are the fastest acting stimulators of glucose uptake, implicating … howes lubricator reviews

Alkaline pH activates the transport activity of GLUT1 in L929 ...

WebMay 13, 2024 · Impairment of cellular glucose uptake due to compromised insulin ... we report the near atomic resolution cryo-EM structures of GLUT4 bound to a small … WebAbstract. Cytochalasin B (17-3 microM) virtually abolished 3-O-methyl-D- [U-14C]glucose uptake and D- [5-3H]glucose utilization in tumoral insulin-producing cells of the RINm5F … WebNov 2, 2011 · Breast cancer cells have a high level of glucose uptake and metabolism, a common characteristic of most cancer cells. This increased glucose uptake and aerobic metabolism forms the basis for assessment of tumor metabolism and response to therapy by positron emission tomography (PET) imaging with FDG (F-18 2-fluoro-2′-deoxyglucose) … howes lubricator \u0026 penetrating oil

Glucose uptake in brown fat cells is dependent on mTOR …

Cryo-EM structure of human glucose transporter GLUT4

WebMay 13, 2024 · Insulin stimulates glucose uptake by acutely upregulating GLUT4 surface levels mainly through increased exocytosis of GSVs and decreased endocytosis 33, 34. A single GLUT4 molecule during its... howes lubricator diesel treat 64 ozWebApr 14, 2016 · Glucose uptake for GlcP Se was measured one minute after initiation of transport with 30 μM C 14 -glucose in the presence of 2 mM … hideaway sleeper couch

"WebCytochalasin B is a cell-permeable fungal toxin which binds to the barbed end of actin filaments inhibiting both the association and dissociation of subunits. It causes the disruption of actin filaments and inhibition of actin polymerization. ... Effect of cytochalasin B on glucose uptake, utilization, oxidation and insulinotropic action in ... " - Cytochalasin b glucose uptake

Cytochalasin b glucose uptake

Cytochalasin B: Inhibition of Glucose and Glucosamine Transport

WebA mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy- d -glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is achieved at … WebThis dependence results in up-regulation of glucose transporter expression, especially hGLUT1. The bound inhibitors include the natural compound cytochalasin B and two of a series of previously undescribed organic compounds that bind in the submicromolar range.

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WebCytochalasin B (1 mg; Sigma-Aldrich; C6762). Add 2 ml DMSO into a vial with 1 mg cytochalasin B (500 μg; 100× CB stock solution). Divide into small tubes (10–20 μl) and store at − 80 °C. • TSA (1 mg; Sigma-Aldrich; T8552). WebCytochalasin B (50µM) was added to determine assay background. Glucose Uptake-Glo™ Assay We have developed a luminescent glucose uptake assay that is based on the same principle as the aforementioned …

WebApr 7, 2024 · It might be related to the F-actin disruption caused by ES, since the distribution of GLUT1 on cell surface could be changed from clusters to dispersed states when the actin cytoskeleton depolymerized by cytochalasin B. We further examined the glucose uptake capacity of cells after the ES using fluorescence glucalogue (2-NBDG), which has the ... WebMay 13, 2024 · A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo. ... To examine the effect of cytochalasin B ...

WebNov 11, 2008 · Cytochalasin B (10 μmol/L) and phloretin (20 μmol/L) inhibited glucose uptake 85 ± 1% and 84 ± 18%, respectively. In contrast, the selective GLUT4 inhibitor … WebIn erythrocytes, adipocytes, and chick embryo fibroblasts, the Ki for glucose uptake inhibition and the Kd for cytochalasin B binding range from 0.1 to 0.4 µM. Values for hepatocytes and liver membranes are an order of magnitude higher …

WebHepG2 cells treated with agents affecting glucose uptake/metabolism (phloretin, quercetin, quercetin-3′-sulfate, NaF, 3-bromopyruvate, NaN3, oligomycin A, ochratoxin A, cytochalasin B, and anti-GLUT1 antibody) showed dose-dependent changes in glucose and ATP levels without total protein (cell) loss. Finally, we performed flow cytometric ...

WebApr 23, 2015 · Moreover, cytochalasin B, a nonspecific glucose transport inhibitor, also strongly inhibited the uptake of 14 C-glucose, 14 C-2DG and 2DG-IR in neuronal and astrocytic cultures . hideaways lake districtWebIn vitro and in vivo study of phloretin-induced apoptosis in human liver cancer cells involving inhibition of type II glucose transporter. Chih Hsiung Wu, Yuan Soon Ho, Chia Yi Tsai, Ying Jan Wang, How Tseng, Po Li Wei, Chia Hwa Lee, Ren Shyan Liu, Shyr Yi Lin. howes lubricator and penetrating oilWebMar 1, 1990 · Glucose uptake in muscle occurs by a system of facilitated diffusion involving at least two distinct glucose transporters, GLUT-1 and GLUT-4. Both bind the fungal … hideaway slaithwaiteWebJun 18, 2024 · The glucose transporter GLUT1, a plasma membrane protein that mediates glucose homeostasis in mammalian cells, is responsible for constitutive uptake of glucose into many tissues and organs. Many studies have focused on its vital physiological functions and close relationship with diseases. hideaway small animalWebMay 1, 1972 · Abstract. A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-d-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, … hide away slimline ironing boardWebFeb 1, 2013 · RBCs from Atlantic salmon showed a linear relationship between glucose uptake and extracellular glucose level, but exhibited clear inhibition of glucose metabolism by cytochalasin B and phloretin, suggesting a component of facilitated glucose transport that is more elusive to detect. hideaway sliding glass doorsWebA novel phosphoinositolglycan-peptide (PIG-P) from the yeast Saccharomyces cerevisiae potently mimicks insulin action on glucose transport and metabolism in rat muscle and adipose tissue. The aim of the present study was to elucidate the cellular howes lubricator diesel treat msds