Dsred express2
WebDsRed-Express2 679 .. 1356 = 678 bp 225 amino acids = 25.7 kDa. Product: noncytotoxic tetrameric variant of DsRed fluorescent protein. mammalian codon-optimized: ori 3987 .. … WebFeb 1, 2024 · DsRed-Express2 and E2-Crimson coding sequences were amplified directly from plasmid DNA. Using these methods, a series of plasmids expressing various fluorescent proteins driven by P c or P psbA promoters was constructed. In some plasmids, fluorescent protein coding sequences were appended to coding sequences for TorT or …
Dsred express2
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WebJul 26, 2024 · C-terminally eGFP-tagged human ZIP14 in pEGFP-N3 vector was purchased from Addgene (Plasmid #104380). Human ZIP14 was PCR amplified without the eGFP tag and subcloned into the pIRES2 DsRed-Express2 vector (Takara Clontech). Transient transfection. HEK293T cells were seeded to achieve an 80 % of cell confluency on the … WebpIRES2 DsRed-Express 2 is a eukaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV) between the MCS and red fluorescent protein DsRed-Express2 coding region.This permits both the gene of …
WebDec 2, 2008 · aggregate, resulting in reduced cyto- and phototoxicity, and making DsRed-Express2 much better suited for . in vivo. applications involving sensitive cells, such as … http://pharmbiology.com/page9?product_id=642
WebDsRed-Express2 is a basic (constitutively fluorescent) orange fluorescent protein published in 2008, derived from Discosoma sp.. It is reported to be a rapidly-maturing protein. We would like to show you a description here but the site won’t allow us. Allison Berman - DsRed-Express2 :: Fluorescent Protein Database Wen Tao - DsRed-Express2 :: Fluorescent Protein Database Hal E Broxmeyer - DsRed-Express2 :: Fluorescent Protein Database References and fluorescent proteins attributed to Dibyendu Bhattacharyya at … Browse. If you just want to browse the database without searching something … A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell … References and fluorescent proteins attributed to Daniel E Strongin at … A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell … WebDec 5, 2005 · Directed evolution of DsRed-Express2 yielded the color variants E2-Orange, E2-Red/Green, and E2-Crimson. These variants can be used to label whole cells for single- and multi-color experiments ...
WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA …
WebdsRED-express2 eGFP. Use. Cre/Lox and Lentiviral Tags. Expression. Mammalian . Mutation. Promoter. CMV. Availability. Academic Institutions and Nonprofits only Enlarge These results may also be relevant. pAC572. Plasmid #127973. Purpose. AMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI) ... nissan road hazard consumer brochureWebApr 3, 2009 · Background: Whole-cell labeling is a common application of fluorescent proteins (FPs), but many red and orange FPs exhibit cytotoxicity that limits their use as whole-cell labels. Recently, a tetrameric red FP called DsRed-Express2 was engineered for enhanced solubility and was shown to be noncytotoxic in bacterial and mammalian cells. nissan rogue 2012 owners manualWebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. nuremberg istanbul flightsWebPlasmid pcDNA3 DSred express2 from Dr. Oskar Laur's lab. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. nissan rogue 2009 roof rackWebDsRed-Express2 is not as bright as wild-type DsRed, so we mutagenized it to create DsRed-Max, which was B30% brighter than DsRed-Express2 and retained high solubility (Fig. 1b and Supplementary Results ). DsRed-Max had lower green emission than DsRed-Express2 (Supplementary Fig. 6 online), but was slower to mature and more photolabile … nissan rogue 2008 headlightsWebDec 21, 2024 · The DsRed-Express2 fluorescent protein provides an effective means for visualizing and quantifying adherent library members as an alternative method to indirect binding assays. Similar co-expression strategies with fluorescent proteins and substrate-specific peptides could also be employed for Förster resonance energy transfer (FRET) … nissan rogue 2011 owners manualWebMar 31, 2024 · HmlΔ-dsRed: a marker for mature hemocytes including both plasmatocytes and crystal cells. Eater-dsRed: a marker that is expressed weakly in distal blood progenitors committed to a plasmaotcyte cell fate but is expresssed strongly in mature plasmatocytes. Lozenge: a crystal cell-specific marker. nissan rogue 2010 headlights