T4 ligase hc

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August undefined, 2024

WebT4 DNA Ligase can be used to efficiently join blunt and cohesive ends and for the repair of single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. The enzyme catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. T4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky-end ligation is completed in 10 minutes at room temperature • Supplied with PEG solution for efficient blunt-end ligation Applications

T4 DNA Ligase Ligase DNA Ligation - Promega

WebT4 DNA Ligase (HC), 500u. M1794 PDF (220 KB) – English (United States) http://www.enzymatics.com/pdf/L603-HC-L.pdf think mutual bank online banking

Can T4 DNA Ligase be heat inactivated? NEB

WebJun 8, 2016 · High performance of T4 ligase is depending on integrity of the ATP in buffer. to keep the balance between T4 ligase optimal activity temperature (25 c) and ATP hydrolysis issue also cross... http://www.enzymatics.com/pdf2/L6030-LC-L.pdf WebB6030 (10X T4 DNA Ligase Buffer) 10X T4 DNA Ligase Buffer (B6030) 500mM Tris-HCI 100 mM MgCl 2 50 mM DTT 10 mM ATP pH 7.6 @ 25°C Unit Definition 1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X T4 DNA Ligase Buffer following a 30 minute incubation at … think mutual bank hours

T4 DNA Ligase (Rapid) The enzyme efficiently joins blunt

Web10 µL 2X Rapid Ligase Buffer X Vector (1 -10 ng/µL ) Y Insert (1 -10 ng/µL ) 1 µL T4 DNA Ligase ( 10 U/ µL) To 20 µL Nuclease -free Water . 2. Mix well by pipetting. 3. Incubate at 25 °C for 10 minutes 4. Purify DNA using a PCR clean-up column and elute in ~50 µL. – OR – Immediately dilute in TE or water (at least 1:10). think mutual bank phone numberWebT4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5' phosphate and 3' hydroxyl termini in double-stranded DNA using ATP as a coenzyme. think mutual bank routing number

"WebApr 14, 2024 · The products were ligated with T4 DNA ligase (TaKaRa, Dalian, Liaoning, China) at 16 °C o/n. The recombinant plasmids were confirmed by Sanger sequencing (Sangon Biotech, Shanghai, China) and co-transfected with pRL-TK plasmid (Renilla luciferase). After 24 h incubation, luciferase activities were measured with Dual … " - T4 ligase hc

T4 ligase hc

T4 DNA Ligase Ligase DNA Ligation - Promega

WebT4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl 2, 100mM DTT and 10mM ATP. ... T4 DNA Ligase (HC) M179A: 1 × 500u: … WebSource of Protein: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene. Unit Definition: 1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C. Molecular weight: 55,292 Daltons. Quality Control ...

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WebT4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates. WebCatalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single ...

WebT4 DNA ligase catalyzes the formation of phosphodiester bonds between adjacent 3′-hydroxyl and 5′-phosphaste termini in double-stranded DNA. The ligase commercially available is now made by recombinant DNA means. T4 DNA ligase is a single polypeptide of 68 kDa; the enzyme requires Mg 2+ and ATP as cofactors. WebT4 DNA Ligase (Rapid) Description [600,000 Units/ml] 240,000 Units (0.40 ml) Share your knowledge of this product with other customers... Be the first to write a review Browse for more products in the same category as this item: Ultrapure Ligases

WebMar 30, 2024 · The key point is the design of L-RNA substrate with appropriate reactivity for T4 Rnl2 ligation, which is efficient enough for intramolecular circularization but not reactive for intermolecular ligation. First, the secondary structure of target C-RNA is determined by Mfold prediction ( 40, 41 ). WebT4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky …

WebThe T4 DNA Ligase requires ATP as a cofactor. Highlights. Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) Fast – sticky …

WebT4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The … think mutual bank online loginWebSep 21, 2016 · So for T4 DNA ligase the catalytic activity at room temperature might be less than when compared to 16 degree celsius. The time also has a role to play as if you don't incubate longer you are... think my dog\u0027s a democratWebGlycerol-Free T4 DNA Ligase (HC) is a glycerol-free, high concentration enzyme (50U/µL) that catalyzes the formation of a phosphodiester bond between adjacent 5ʹ phosphate and 3' hydroxyl groups in duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. think my dog had a strokeWebRNA Circularization using T4 RNA Ligase 1 Protocol for ssDNA/RNA Ligation (M0319) Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1 3´ End Labeling of RNA using T4 RNA Ligase 1 Typical Protocol for RNA Ligation (M0458) Featured Products T4 RNA Ligase 2, truncated K227Q T4 RNA Ligase 1 (ssRNA Ligase) think mutual bank rochester mn addressWebLigation Protocol with T4 DNA Ligase (M0202) Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the … think my dog is depressedWebT4 RNA Ligase 1 Add to Cart: L6050L: 10,000 U: 20,000 U/ml: $443: Single-stranded RNA ligase. Also joins single-stranded DNA molecules. Add to Cart: T4 DNA Ligase (Rapid) Add to Cart: L6030-HC-L: 240,000 U: 600,000 U/ml: $707 “Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008). Add to Cart: T3 DNA Ligase Add to Cart ... think my driverWebFollowing the amplification, the amplified L arm and R arm fragments were phosphorylated by T4 polynucleotide kinase (PNK, New England Biolabs) during 30 min at 37 °C and then ligated with each other by T4 ligase (Thermo-Scientific). The final total fragment was cloned into the vector pJet1.2 (Thermo-Scientific). think my house is haunted